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World Health Organization : Regioinal Office for Africa ; Year 1993 ; Africa Region, 6403, Ex.196, No. 03.05: Prevention of Water Borne Diseases through the Quality Control of Water

By World Health Organization

Medical Reference Publication

As indicated in Table I, RPMI and F12 media were superior to the two 199 media tested when 10% pooled human serum (PHS) was used. In addition, exhaustively dialysed human serum was not effective when used to supplement RPMI, but was not so poor when used with F12 medium and the two 199 media. When human serum was dialysed against F12 medium and then used to supplement RPMI it remained effective. Since Jensen (3) has previously shown that dialysis of human serum results in the loss of factors of low relative molecular mass (less than 6000- 8000 RMM) which are essential for parasite growth, these results indicate that El2 and the 199 media appear to contain factors that promote parasite growth which are not present in RPMI. Because El2 appeared to be the better medium under these conditions it was used to make further comnarisons. The factors present in F12 but not in R P M ~we re used to supplement RPMI.

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