World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, And Cdcl2

By Somji, Seema

Click here to view

Book Id: WPLBN0000226155
Format Type: PDF eBook
File Size: 0.3 MB
Reproduction Date: 2005

Title: Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, And Cdcl2  
Author: Somji, Seema
Volume:
Language: English
Subject: Government publications, United Nations., United Nations. Office for Disarmament Affairs
Collections: Government Library Collection, Disarmament Documents
Historic
Publication Date:
Publisher: United Nations- Office for Disarmament Affairs (Unoda)

Citation

APA MLA Chicago

Somji, S. (n.d.). Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, And Cdcl2. Retrieved from http://www.ebooklibrary.org/


Description
Government Reference Publication

Excerpt
Excerpt: We determined the expression of the constitutive (hsc 70) and inducible (hsp 70) forms of heat shock protein 70 mRNA and protein in human proximal tubule (HPT) cells exposed to lethal and sublethal concentrations of Cd+2 under both acute and extended conditions of exposure. The HPT cells exhibited the classic heat shock response when subjected to a physical (heat) or chemical stress (sodium arsenite); hsc 70 mRNA and protein levels were constant or slightly increased, whereas hsp 70 mRNA and protein were greatly elevated. Acute exposure to 53.4 micrometer CdCl2 for 4 hr failed to increase either hsc 70 mRNA or protein, a finding similar to that observed under classic conditions of stress. However, under identical conditions of acute exposure to Cd2+, the expected increase in hsp 70 protein level was suppressed as compared to that found under classic conditions of physical or chemical stress. The decrease in hsp 70 protein level correlated to the reduced expression of mRNA from the hsp 70B gene. The expression of mRNA from the hsp 70A and hsp 70C genes was similar to that found when the cells were treated with heat shock or sodium arsenite. We modeled an extended exposure to Cd2+ by treating the cells continuously with Cd2+ at both lethal and sublethal levels over a 16-day time course. Chronic exposure to Cd2+ failed to increase either hsc 70 mRNA or protein levels in the HPT cells at a nonlethal dosage level and decreased hsc 70 mRNA and protein levels late in the time course of lethal exposure. Under identical conditions, the expression of hsp 70 protein remained at basal levels that were only marginally detectable throughout the time course. Hsp 70A and hsp 70C mRNA levels were unaltered by extended exposure to Cd2+, and hsp 70B mRNA was not detected during the 16-day time course. Cd2+ is a poor inducer of hsc 70 and hsp 70 in the proximal tubule under both acute and long-term exposure. These results reinforce the fact that the expression of hsp 70 protein does not result from the transcription of a single gene, but is derived from what may be a complex interplay of several underlying genes. Key words: cadmium, gene expression, heat shock, heavy metals, hsc 70, hsp 70, proximal tubule, sodium arsenite. Environ Health Perspect 107:887?893 (1999). [12 October 1999].


 

Click To View

Additional Books


  • Afrikaans to English Dictionary (by )
  • The Noble Koran (Quran) : The Cow (by )
  • The Journal of the College of Science, I... (by )
  • Engineering Thermodynamics (by )
  • Guatimoc Guatimocin : Tragedia en Cinco ... (by )
  • Commercial News (by )
  • Brain and Personality; Or, The Physical ... (by )
  • The Philosophical Strangler (by )
  • The 2005 National Export Strategy (by )
  • Mine Accounts and Mining Book-Keeping : ... (by )
  • The Two Eids and Their Significance (by )
  • The Koran (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.