World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : No Influence of Indy on Lifespan in Drosophila After Correction for Genetic and Cytoplasmic Background Effects, Volume 3

By Kim, Stuart K.

Click here to view

Book Id: WPLBN0003924608
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : No Influence of Indy on Lifespan in Drosophila After Correction for Genetic and Cytoplasmic Background Effects, Volume 3  
Author: Kim, Stuart K.
Volume: Volume 3
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection, PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Kim, S. K. (n.d.). Plos Genetics : No Influence of Indy on Lifespan in Drosophila After Correction for Genetic and Cytoplasmic Background Effects, Volume 3. Retrieved from http://www.ebooklibrary.org/


Description
Description : To investigate whether alterations in mitochondrial metabolism affect longevity in Drosophila melanogaster, we studied lifespan in various single gene mutants, using inbred and outbred genetic backgrounds. As positive controls we included the two most intensively studied mutants of Indy, which encodes a Drosophila Krebs cycle intermediate transporter. It has been reported that flies heterozygous for these Indy mutations, which lie outside the coding region, show almost a doubling of lifespan. We report that only one of the two mutants lowers mRNA levels, implying that the lifespan extension observed is not attributable to the Indy mutations themselves. Moreover, neither Indy mutation extended lifespan in female flies in any genetic background tested. In the original genetic background, only the Indy mutation associated with altered RNA expression extended lifespan in male flies. However, this effect was abolished by backcrossing into standard outbred genetic backgrounds, and was associated with an unidentified locus on the X chromosome. The original Indy line with long-lived males is infected by the cytoplasmic symbiont Wolbachia, and the longevity of Indy males disappeared after tetracycline clearance of this endosymbiont. These findings underscore the critical importance of standardisation of genetic background and of cytoplasm in genetic studies of lifespan, and show that the lifespan extension previously claimed for Indy mutants was entirely attributable to confounding variation from these two sources. In addition, we saw no effects on lifespan of expression knockdown of the Indy orthologues nac-2 and nac-3 in the nematode Caenorhabditis elegans.

 

Click To View

Additional Books


  • Plos Genetics : Enrichment of Hp1A on Dr... (by )
  • Plos Genetics : Distinct Cdk1 Requiremen... (by )
  • Plos Genetics : Comparative Analysis of ... (by )
  • Plos Genetics : Genome-wide Patterns of ... (by )
  • Plos Genetics : Dynamic Large-scale Chro... (by )
  • Plos Genetics : Widespread Hypomethylati... (by )
  • Plos Genetics : Consequences of Lineage-... (by )
  • Plos Genetics : a Molecular Phylogeny of... (by )
  • Plos Genetics : Offspring’s Leukocyte Te... (by )
  • Plos Genetics : Role of the Single-stran... (by )
  • Plos Genetics : Early Endocardial Morpho... (by )
  • Plos Genetics : Cell Polarity and Patter... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.