World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : the in Vivo Kinetics of Rna Polymerase II Elongation During Co-transcriptional Splicing, Volume 9

By Public Library of Science (Plos)

Click here to view

Book Id: WPLBN0003952337
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : the in Vivo Kinetics of Rna Polymerase II Elongation During Co-transcriptional Splicing, Volume 9  
Author: Public Library of Science (Plos)
Volume: Volume 9
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Biology
Publication Date:
Publisher: Plos


APA MLA Chicago

Of Science (Plos), P. L. (n.d.). Plos Biology : the in Vivo Kinetics of Rna Polymerase II Elongation During Co-transcriptional Splicing, Volume 9. Retrieved from

Description : RNA processing events that take place on the transcribed pre-mRNA include capping, splicing, editing, 39 processing, and polyadenylation. Most of these processes occur co-transcriptionally while the RNA polymerase II (Pol II) enzyme is engaged in transcriptional elongation. How Pol II elongation rates are influenced by splicing is not well understood. We generated a family of inducible gene constructs containing increasing numbers of introns and exons, which were stably integrated in human cells to serve as actively transcribing gene loci. By monitoring the association of the transcription and splicing machineries on these genes in vivo, we showed that only U1 snRNP localized to the intronless gene, consistent with a splicing-independent role for U1 snRNP in transcription. In contrast, all snRNPs accumulated on intron-containing genes, and increasing the number of introns increased the amount of spliceosome components recruited. This indicates that nascent RNA can assemble multiple spliceosomes simultaneously. Kinetic measurements of Pol II elongation in vivo, Pol II ChIP, as well as use of Spliceostatin and Meayamycin splicing inhibitors showed that polymerase elongation rates were uncoupled from ongoing splicing. This study shows that transcription elongation kinetics proceed independently of splicing at the model genes studied here. Surprisingly, retention of polyadenylated mRNA was detected at the transcription site after transcription termination. This suggests that the polymerase is released from chromatin prior to the completion of splicing, and the pre-mRNA is post-transcriptionally processed while still tethered to chromatin near the gene end.


Click To View

Additional Books

  • Plos Biology : Vitamin Supplements Impro... (by )
  • Plos Biology : Replication Publication, ... (by )
  • Plos Biology : Transcriptional Corepress... (by )
  • Plos Biology : Antigen-specific T Cells ... (by )
  • Plos Biology : Structural Polymorphism o... (by )
  • Plos Biology : Neuronal Oscillations Enh... (by )
  • Plos Biology : Secondary Evolution of a ... (by )
  • Plos Biology : an Inordinate Fondness fo... (by )
  • Plos Biology : a Role for the Period;Per... (by )
  • Plos Biology : Dissecting the Urge to Cr... (by )
  • Plos Biology : Invaders on the Wing, Vol... (by )
  • Plos Biology : Evolutionarily Conserved ... (by )
Scroll Left
Scroll Right


Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.