World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Alternative Oxidase Expression in the Mouse Enables Bypassing Cytochrome C Oxidase Blockade and Limits Mitochondrial Ros Overproduction, Volume 9

By Larsson, Nils-göran

Click here to view

Book Id: WPLBN0003952783
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Alternative Oxidase Expression in the Mouse Enables Bypassing Cytochrome C Oxidase Blockade and Limits Mitochondrial Ros Overproduction, Volume 9  
Author: Larsson, Nils-göran
Volume: Volume 9
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Larsson, N. (n.d.). Plos Genetics : Alternative Oxidase Expression in the Mouse Enables Bypassing Cytochrome C Oxidase Blockade and Limits Mitochondrial Ros Overproduction, Volume 9. Retrieved from http://www.ebooklibrary.org/


Description
Description : Cyanide-resistant non-phosphorylating respiration is known in mitochondria from plants, fungi, and microorganisms but is absent in mammals. It results from the activity of an alternative oxidase (AOX) that conveys electrons directly from the respiratory chain (RC) ubiquinol pool to oxygen. AOX thus provides a bypath that releases constraints on the cytochrome pathway and prevents the over-reduction of the ubiquinone pool, a major source of superoxide. RC dysfunctions and deleterious superoxide overproduction are recurrent themes in human pathologies, ranging from neurodegenerative diseases to cancer, and may be instrumental in ageing. Thus, preventing RC blockade and excess superoxide production by means of AOX should be of considerable interest. However, because of its energy-dissipating properties, AOX might produce deleterious effects of its own in mammals. Here we show that AOX can be safely expressed in the mouse (MitAOX), with major physiological parameters being unaffected. It neither disrupted the activity of other RC components nor decreased oxidative phosphorylation in isolated mitochondria. It conferred cyanide-resistance to mitochondrial substrate oxidation and decreased reactive oxygen species (ROS) production upon RC blockade. Accordingly, AOX expression was able to support cyanide-resistant respiration by intact organs and to afford prolonged protection against a lethal concentration of gaseous cyanide in whole animals. Taken together, these results indicate that AOX expression in the mouse is innocuous and permits to overcome a RC blockade, while reducing associated oxidative insult. Therefore, the MitAOX mice represent a valuable tool in order to investigate the ability of AOX to counteract the panoply of mitochondrialinherited diseases originating from oxidative phosphorylation defects.

 

Click To View

Additional Books


  • Plos Genetics : Deleterious Alleles in t... (by )
  • Plos Genetics : Ccdc94 Protects Cells fr... (by )
  • Plos Genetics : Molecular Evolution and ... (by )
  • Plos Genetics : Protein Under-wrapping C... (by )
  • Plos Genetics : Network Properties of Ro... (by )
  • Plos Genetics : Imputation-based Analysi... (by )
  • Plos Genetics : Positional Cloning Revea... (by )
  • Plos Genetics : Ku Regulates the Non-hom... (by )
  • Plos Genetics : Genomic Variation in Ric... (by )
  • Plos Genetics : Regulatory Cross-talk Li... (by )
  • Plos Genetics : Characterization of Line... (by )
  • Plos Genetics : Transcriptome Analysis o... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.