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Plos One : Probing the Binding Sites of Antibiotic Drugs Doxorubicin and N-trifluoroacetyl Doxorubicin with Human and Bovine Serum Albumins, Volume 7

By Sem, Daniel, S.

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Book Id: WPLBN0003958714
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Probing the Binding Sites of Antibiotic Drugs Doxorubicin and N-trifluoroacetyl Doxorubicin with Human and Bovine Serum Albumins, Volume 7  
Author: Sem, Daniel, S.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

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Sem, D. S. (n.d.). Plos One : Probing the Binding Sites of Antibiotic Drugs Doxorubicin and N-trifluoroacetyl Doxorubicin with Human and Bovine Serum Albumins, Volume 7. Retrieved from http://www.ebooklibrary.org/


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Description : We located the binding sites of doxorubicin (DOX) and N-(trifluoroacetyl) doxorubicin (FDOX) with bovine serum albumin (BSA) and human serum albumins (HSA) at physiological conditions, using constant protein concentration and various drug contents. FTIR, CD and fluorescence spectroscopic methods as well as molecular modeling were used to analyse drug binding sites, the binding constant and the effect of drug complexation on BSA and HSA stability and conformations. Structural analysis showed that doxorubicin and N-(trifluoroacetyl) doxorubicin bind strongly to BSA and HSA via hydrophilic and hydrophobic contacts with overall binding constants of KDOX-BSA = 7.8 (60.7)6103 M21, KFDOX-BSA = 4.8 (60.5)6103 M21 and KDOX-HSA = 1.1 (60.3)6104 M21, KFDOX-HSA = 8.3 (60.6)6103 M21. The number of bound drug molecules per protein is 1.5 (DOX-BSA), 1.3 (FDOX-BSA) 1.5 (DOX-HSA), 0.9 (FDOX-HSA) in these drug-protein complexes. Docking studies showed the participation of several amino acids in drug-protein complexation, which stabilized by H-bonding systems. The order of drug-protein binding is DOX-HSA . FDOX-HSA . DOX-BSA . FDOX.BSA. Drug complexation alters protein conformation by a major reduction of a-helix from 63% (free BSA) to 47–44% (drug-complex) and 57% (free HSA) to 51–40% (drug-complex) inducing a partial protein destabilization. Doxorubicin and its derivative can be transported by BSA and HSA in vitro.

 

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