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Plos One : Temperature-triggered Enzyme Immobilization and Release Based on Cross-linked Gelatin Nanoparticles, Volume 7

By Shankar, Sangaru Shiv

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Book Id: WPLBN0003960660
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Temperature-triggered Enzyme Immobilization and Release Based on Cross-linked Gelatin Nanoparticles, Volume 7  
Author: Shankar, Sangaru Shiv
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

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Shankar, S. S. (n.d.). Plos One : Temperature-triggered Enzyme Immobilization and Release Based on Cross-linked Gelatin Nanoparticles, Volume 7. Retrieved from http://www.ebooklibrary.org/


Description
Description : A glucoamylase-immobilized system based on cross-linked gelatin nanoparticles (CLGNs) was prepared by coacervation method. This system exhibited characteristics of temperature-triggered phase transition, which could be used for enzyme immobilization and release. Their morphology and size distribution were examined by transmission electron microscopy and dynamic light scattering particle size analyzer. Their temperature-triggered glucoamylase immobilization and release features were also further investigated under different temperatures. Results showed that the CLGNs were regularly spherical with diameters of 15565 nm. The loading efficiencies of glucoamylase immobilized by entrapment and adsorption methods were 59.9% and 24.7%, respectively. The immobilized enzyme was released when the system temperature was above 40uC and performed high activity similar to free enzyme due to the optimum temperature range for glucoamylase. On the other hand, there was no enzyme release that could be found when the system temperature was below 40uC. The efficiency of temperature-triggered release was as high as 99.3% for adsorption method, while the release of enzyme from the entrapment method was not detected. These results indicate that CLGNs are promising matrix for temperature-triggered glucoamylase immobilization and release by adsorption immobilization method.

 

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