World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : the Rna Degradation Pathway Regulates the Function of Gas5 a Non-coding Rna in Mammalian Cells, Volume 7

By Kanai, Akio

Click here to view

Book Id: WPLBN0003961428
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : the Rna Degradation Pathway Regulates the Function of Gas5 a Non-coding Rna in Mammalian Cells, Volume 7  
Author: Kanai, Akio
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Kanai, A. (n.d.). Plos One : the Rna Degradation Pathway Regulates the Function of Gas5 a Non-coding Rna in Mammalian Cells, Volume 7. Retrieved from http://www.ebooklibrary.org/


Description
Description : Studies of various mRNAs have revealed that changes in the abundance of transcripts, through mRNA degradation, act as a critical step in the control of various biological pathways. Similarly, the regulation of non-coding RNA (ncRNA) levels is also considered to be important for their biological functions: however, far less is known about the mechanisms and biological importance of ncRNA turnover for the regulation of ncRNA functions. The growth arrest-specific 5 (GAS5) ncRNA accumulates during growth arrest induced by serum starvation and its transcript is degraded by the well characterized nonsense-mediated RNA decay (NMD) pathway. Historically, NMD was discovered as a RNA quality control system to eliminate aberrant transcripts: however, accumulating evidence shows that NMD also regulates the abundance of physiological transcripts. Interestingly, the GAS5 transcript has the ability to bind the glucocorticoid receptor (GR), resulting in the inhibition of its ligand-dependent association with DNA. The GR binds the promoters of various glucocorticoidresponsive genes, including apoptosis-related genes. In this study, we examined whether the RNA degradation pathway can regulate this function of GAS5. We measured the steady-state abundance and the decay rate of GAS5 in UPF1-depleted human cells using the 59-bromo-uridine immunoprecipitation chase (BRIC) method, an inhibitor-free method for directly measuring RNA stability. We found that levels of the GAS5 transcript were elevated owing to prolonged decay rates in response to UPF1 depletion, and consequently the apoptosis-related genes, cIAP2 and SGK1, were down-regulated. In addition, serum starvation also increased the transcript levels of GAS5 because of prolonged decay rates, and conversely decreased levels of cIAP2 and SGK1 mRNA. Taken together, we found that the RNA degradation pathway can regulate the function of the GAS5 ncRNA in mammalian cells.

 

Click To View

Additional Books


  • Plos One : Effect of Dielectric and Liqu... (by )
  • Plos One : Nef-specific Cd8+ T Cell Resp... (by )
  • Plos One : Tempol Moderately Extends Sur... (by )
  • Plos One : Investigation of Cd28 Gene Po... (by )
  • Plos One : Biological Characterization o... (by )
  • Plos One : Specific Targeting of Caspase... (by )
  • Plos One : the P50 Subunit of Nf-kb Orch... (by )
  • Plos One : Sleep Loss and Cytokines Leve... (by )
  • Plos One : Pkg II Inhibits Egf, Volume 8 (by )
  • Plos One : Diversity and Complexity in C... (by )
  • Plos One : Heterogeneity in the Energy C... (by )
  • Plos One : a Comparison of Structural an... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.