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Activity and Diversity of Methane-oxidizing Bacteria in Glacier Forefields on Siliceous and Calcareous Bedrock : Volume 9, Issue 1 (30/01/2012)

By Nauer, P. A.

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Book Id: WPLBN0003973550
Format Type: PDF Article :
File Size: Pages 40
Reproduction Date: 2015

Title: Activity and Diversity of Methane-oxidizing Bacteria in Glacier Forefields on Siliceous and Calcareous Bedrock : Volume 9, Issue 1 (30/01/2012)  
Author: Nauer, P. A.
Volume: Vol. 9, Issue 1
Language: English
Subject: Science, Biogeosciences, Discussions
Collections: Periodicals: Journal and Magazine Collection (Contemporary), Copernicus GmbH
Publication Date:
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications


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Liesack, W., Dam, B., Nauer, P. A., Schroth, M. H., & Zeyer, J. (2012). Activity and Diversity of Methane-oxidizing Bacteria in Glacier Forefields on Siliceous and Calcareous Bedrock : Volume 9, Issue 1 (30/01/2012). Retrieved from

Description: Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, 8092 Zurich, Switzerland. The global methane (CH4) cycle is largely driven by methanogenic archaea and methane-oxidizing bacteria (MOB), but little is known about their activity and diversity in pioneer ecosystems. We conducted a field survey in forefields of 13 receding Swiss glaciers on both siliceous and calcareous bedrock to investigate and quantify CH4 turnover based on soil-gas CH4 concentration profiles, and to characterize MOB communities using pmoA sequencing and T-RFLP. Methane turnover was fundamentally different in the two bedrock categories. Of the 36 CH4 concentration profiles from siliceous locations, 11 showed atmospheric CH4 consumption at concentrations of ∼1–2 μl l−1 with soil-atmosphere CH4 fluxes of −0.14 to −1.1 mg m−2 d−1. Another 11 profiles showed no apparent activity, while the remaining 14 exhibited slightly increased CH4 concentrations of ∼2–10 μl l−1, most likely due to microsite methanogenesis. In contrast, all profiles from calcareous sites suggested a substantial, yet unknown CH4 source below our sampling zone, with soil-gas CH4 concentrations reaching up to 1400 μl l−1. Remarkably, most soils oxidized ∼90% of the deep-soil CH4, resulting in soil-atmosphere fluxes of 0.12 to 31 mg m−2 d−1. MOB showed limited diversity in both siliceous and calcareous forefields: all identified pmoA sequences formed only 5 OTUs and, with one exception, could be assigned to either Methylocystis or the as-yet-uncultivated Upland Soil Cluster Γ (USCΓ). The latter dominated T-RFLP patterns of all siliceous and most calcareous samples, while Methylocystis dominated in 4 calcareous samples. As Type I MOB are widespread in cold climate habitats with elevated CH4 concentrations, USCΓ might be the corresponding Type I MOBs in habitats exposed to near-atmospheric CH4 concentrations.

Activity and diversity of methane-oxidizing bacteria in glacier forefields on siliceous and calcareous bedrock

Adamsen, A. P. S. and King, G. M.: Methane consumption in temperate and subarctic forest soils: Rates, vertical zonation, and responses to water and nitrogen, Appl. Environ. Microb., 59, 485–490, 1993.; Andersen, B. L., Bidoglio, G., Leip, A., and Rembges, D.: A new method to study simultaneous methane oxidation and methane production in soils, Global Biogeochem. Cy., 12, 587–594, 1998.; Auman, A. J., Speake, C. C., and Lidstrom, M. E.: nifH sequences and nitrogen fixation in type I and type II methanotrophs, Appl. Environ. Microb., 67, 4009–4016, 2001.; Baani, M. and Liesack, W.: Two isozymes of particulate methane monooxygenase with different methane oxidation kinetics are found in Methylocystis sp. strain SC2, P. Natl. Acad. Sci. USA, 105, 10203–10208, 2008.; Bárcena, T. G., Yde, J. C., and Finster, K. W.: Methane flux and high-affinity methanotrophic diversity along the chronosequence of a receding glacier in Greenland, Ann. Glaciol., 51, 23–31, 2010.; Bárcena, T., Finster, K., and Yde, J.: Spatial patterns of soil development, methane oxidation, and methanotrophic diversity along a receding glacier forefield, southeast Greenland, Arct. Antarct. Alp. Res., 43, 178–188, 2011.; Bender, M. and Conrad, R.: Kinetics of CH4 oxidation in oxic soils exposed to ambient air or high CH4 mixing ratios, FEMS Microbiol. Lett., 101, 261–270, 1992.; Dunfield, P. F.: The soil methane sink, in: Greenhouse gas sinks, edited by: Reay, D., Hewitt, K., Smith, K., and Grace, J., CABI, Wallingford, 152–170, 2007.; Bernasconi, S. M., Bauder, A., Bourdon, B., Brunner, I., Bünemann, E., Chris, I., Derungs, N., Edwards, P., Farinotti, D., Frey, B., Frossard, E., Furrer, G., Gierga, M., Göransson, H., Gülland, K., Hagedorn, F., Hajdas, I., Hindshaw, R., Ivy-Ochs, S., Jansa, J., Jonas, T., Kiczka, M., Kretzschmar, R., Lemarchand, E., Luster, J., Magnusson, J., Mitchell, E. A. D., Venterink, H. O., Plötze, M., Reynolds, B., Smittenberg, R. H., Stähli, M., Tamburini, F., Tipper, E. T., Wacker, L., Welc, M., Wiederhold, J. G., Zeyer, J., Zimmermann, S., and Zumsteg, A.: Chemical and biological gradients along the Damma glacier soil chronosequence, Switzerland, Vadose Zone J., 10, 867–883, 2011.; Born, M., Dörr, H., and Levin, I.: Methane consumption in aerated soils of the temperate zone, Tellus B, 42, 2–8, 1990.; Bourne, D. G., McDonald, I. R., and Murrell, J. C.: Comparison of pmoA PCR primer sets as tools for investigating methanotroph diversity in three Danish soils, Appl. Environ. Microb., 67, 3802–3809, 2001.; Brankatschk, R., Towe, S., Kleineidam, K., Schloter, M., and Zeyer, J.: Abundances and potential activities of nitrogen cycling microbial communities along a chronosequence of a glacier forefield, ISME J., 5, 1025–1037, 2011.; Conrad, R.: Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO), Microbiol. Rev., 60, 609–640, 1996.; Conrad, R.: The global methane cycle: Recent advances in understanding the microbial processes involved, Environ. Microbiol. Reports, 1, 285–292, 2009.; Costello, A. M. and Lidstrom, M. E.: Molecular characterization of functional and phylogenetic genes from natural populations of methanotrophs in lake sediments, Appl. Environ. Microb., 65, 5066–5074, 1999.; De Visscher, A., Boeckx, P., and van Cleemput, O.: Artificial methane sinks, in: Greenhouse gas sinks, edited by: Reay, D. S., Hewitt, C. N., Smith, K. A., and Grace, J., CABI, Wallingford, 184–200, 2007.; Dedysh, S. N., Ricke, P., and Liesack, W.: NifH and NifD phylogenies: an evolutionary basis for understanding nitrogen fixation capabilities of methanotrophic bacteria, Microbiology (SGM), 150, 1301–1313, 2004.; Degelmann, D. M., Borken, W., Drake, H. L., and Kolb, S.: Different atmospheric methane-oxidizing communities in European beec


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