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Molecular Biology Techniques and Applications for Ocean Sensing : Volume 5, Issue 2 (08/05/2009)

By Zehr, J. P.

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Book Id: WPLBN0004020372
Format Type: PDF Article :
File Size: Pages 13
Reproduction Date: 2015

Title: Molecular Biology Techniques and Applications for Ocean Sensing : Volume 5, Issue 2 (08/05/2009)  
Author: Zehr, J. P.
Volume: Vol. 5, Issue 2
Language: English
Subject: Science, Ocean, Science
Collections: Periodicals: Journal and Magazine Collection, Copernicus GmbH
Publication Date:
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications


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Moisander, P., Hewson, I., & Zehr, J. P. (2009). Molecular Biology Techniques and Applications for Ocean Sensing : Volume 5, Issue 2 (08/05/2009). Retrieved from

Description: Department of Ocean Sciences, University of California Santa Cruz, 1156 High Street E&MS D446, Santa Cruz, CA 95064, USA. The study of marine microorganisms using molecular biological techniques is now widespread in the ocean sciences. These techniques target nucleic acids which record the evolutionary history of microbes, and encode for processes which are active in the ocean today. Molecular techniques can form the basis of remote instrumentation sensing technologies for marine microbial diversity and ecological function. Here we review some of the most commonly used molecular biological techniques. These techniques include the polymerase chain reaction (PCR) and reverse-transcriptase PCR, quantitative PCR, whole assemblage fingerprinting approaches (based on nucleic acid sequence or length heterogeneity), oligonucleotide microarrays, and high-throughput shotgun sequencing of whole genomes and gene transcripts, which can be used to answer biological, ecological, evolutionary and biogeochemical questions in the ocean sciences. Moreover, molecular biological approaches may be deployed on ocean sensor platforms and hold promise for tracking of organisms or processes of interest in near-real time.

Molecular biology techniques and applications for ocean sensing

Dyhrman, S. T. and Haley, S. T.: Phosphorus scavenging in the unicellular marine diazotroph \textitCrocosphaera watsonii, Appl. Environ. Microb., 72, 1452–1458, 2006.; % vor jede Referenz Acinas, S. G., Klepac-Ceraj, V., Hunt, D. E., Pharino, C., Ceraj, I., et al.: Fine-scale phylogenetic architecture of a complex bacterial community, Nature, 430, 551–554, 2004.; Agogue, H., Casamayor, E. O., Bourrain, M., Obernosterer, I., Joux, F., et al.: A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems, FEMS Microbiol. Ecol., 54, 269–280, 2005.; Amann, R. I., Krumholz, L., and Stahl, D. A.: Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology, J. Bacteriol., 172, 762–770, 1990.; Angly, F. E., Felts, B., Breitbart, M., Salamon, P., Edwards, R. A., et al.: The marine viromes of four oceanic regions, PLoS Biology, 4, 2121–2131, 2006.; Amann, R. I., Ludwig, W., and Schleifer, K.-H.: Phylogenetic identification and \textitin situ detection of individual microbial cells without cultivation, Microbiol. Rev., 59, 143–169, 1995.; Avaniss-Aghajani, E., Jones, K., Chapman, D., and Brunk, C.: A molecular technique for identification of bacteria using small subunit ribosomal RNA sequences, Biotechniques, 17, 144–149, 1994.; Azam, F.: Microbial control of oceanic carbon flux: The plot thickens, Science, 280, 694–696, 1998.; Barlaan, E. A., Furukawa, S., and Takeuchi, K.: Detection of bacteria associated with harmful algal blooms from coastal and microcosm environments using electronic microarrays, Environ. Microbiol., 9, 690–702, 2007.; Barnes, W. M.: The fidelity of \textitTaq polymerase catalyzing PCR is improved by an N-terminal deletion, Gene, 112, 29–35, 1992.; Béjà, O., Aravind, L., Koonin, E. V., Suzuki, M. T., Hadd, A., et al.: Bacterial rhodopsin: Evidence for a new type of phototrophy in the sea, Science, 289, 1902–1906, 2000.; Bodrossy, L., Stralis-Pavese, N., Murrell, J. C., Radajewski, S., Weilharter, A., et al.: Development and validation of a diagnostic microbial microarray for methanotrophs, Environ. Microbiol., 5, 566–582, 2003.; Braker, G., Ayala-del-Rio, H. L., Devol, A. H., Fesefeldt, A., and Tiedje, J. M.: Community structure of denitrifiers, bacteria, and archaea along redox gradients in Pacific northwest marine sediments by terminal restriction fragment length polymorphism analysis of amplified nitrite reductase (\textitnirS) and 16s~rRna genes, Appl. Environ. Microb., 67, 1893–1901, 2001.; Breitbart, M., Segall, A., Mead, D., Azam, F., and Rohwer, F.: Genomic analysis of an uncultured marine viral community, EOS Transactions, 83, OS11I-02, 2002.; Breitbart, M., Felts, B., Kelley, S., Mahaffy, J. M., Nulton, J., et al.: Diversity and population structure of a near-shore marine-sediment viral community, P. Roy. Soc. Lond. B Bio., 271, 565–574, 2004.; Brown, M. V., Schwalbach, M. S., Hewson, I., and Fuhrman, J. A.: Coupling 16S-ITS rDNA clone libraries and automated ribosomal intergenic spacer analysis to show marine microbial diversity: development and application to a time series, Environ. Microbiol., 7, 1466–1479, 2005.; Burgmann, H., Howard, E. C., Ye, W. Y., Sun, F., Sun, S. L., et al.: Transcriptional response of \textitSilicibacter \textitpomeroyi DSS-3 to dimethylsulfoniopropionate (DMSP), Environ. Microbiol., 9, 2742–2755, 2007.; Casper, E. T., Paul, J. H., Smith, M. C., and Gray, M.: Detection and quantification of the red tide dinoflagellate \textitKarenia \textitbrevis by real-time nucleic acid sequence-based amplification, Appl. Environ. Microb., 70, 4727–4732, 2004.; Castiglioni, B., Rizzi, E., Frosini, A., Sivonen, K., Rajaniemi, P., et al.: Development of a universal microarray based on the ligation detection reaction and 16S rRNA gene polymorphism to target diversity of cyanobacteria, Appl. Environ. Microb., 70, 7161–7172, 2004.; Chao, A.: Non-parametric estimation of the number of classes in a population, Scand.


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